RFLP Analysis of Beta‐Lactoglobulin Gene in Swamp and Murrah Buffaloes Using a Single Restriction Enzyme

An attempt has been made to analyze the distribution of the beta-lactoglobulin genotype in swamp buffaloes and Murrah buffaloes utilizing polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Blood samples were taken from 50 swamp buffaloes and 50 Murrah buffaloes. The DNA was extracted by the phenol-chloroform method. The PCR-RFLP was performed using beta-lactoglobulin gene primers and a single restriction enzyme, Hae III. The enzyme digested products were separated by electrophoresis on 2.5%agarose gel. All the 100 DNA samples of swamp and Murrah buffaloes resulted in 398 bp product on amplifying beta-lactoglobulin gene. Those PCR products (398 bp fragment) were digested with Hae III produced only one type of restriction pattern yielding five fragments of 113, 99, 89, 73 and 24 bp. One hundred DNA samples of swamp and Murrah buffalo were examined in this study and revealed no polymorphism at the beta-lactoglobulin gene locus.